The Macedonia Missionary Baptist Church of Ocean Springs, Mississippi, was organized in 1891. Early meetings were held in a city-owned building until a sanctuary was completed on Weed Street in 1914. The congregation worked for years to retire the church mortgage, with significant fundraising efforts between 1917 and 1920.

In 1898, the church purchased land at Weed Street and what is now M.L. King Jr. Avenue. Additional land on nearby Taylor (formerly Church) Street was acquired in 1915 for a rectory. The church was rebuilt in 1947 with assistance from both Black and white community members.

Reverend James Thompson served until his passing in 1932. In 1968, Reverend Dr. Jesse L. Trotter Sr. became pastor, leading the congregation for more than 40 years. A dedicated educator and community leader, he founded the LIFT program and LIFT Bible Crusade College, served as a Head Start project director, and represented Ward I as an alderman. Under his leadership, a new sanctuary was built in 1998–1999 after the church purchased adjoining lots to expand parking.

Following Dr. Trotter’s death in 2010, Weed Street—where he lived and preached for decades—was renamed Reverend Jesse L. Trotter Sr. Street in his honor. In 2012, Dr. Albert Dantzler Jr. became pastor until his passing in 2013.

Reverend John A. Robinson became pastor on February 1, 2015. A lifelong minister known for preaching and gospel singing, he welcomed 80 new members in his first year and continues to focus on community engagement and spiritual growth.